MAP-Tau Kit
MAP-tau quantification in cell lysates
Total alpha Synuclein cellular kit HTRF®
This HTRF kit detects cellular alpha Synuclein and can be combined with our phospho-alpha Synuclein S129 kit.
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Ready-to-use
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High sensitivity
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Enables fast assay qualification
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No-wash
Overview
The Total alpha Synuclein cellular assay is designed to monitor the expression level of alpha Synuclein proteins, both phosphorylated and unphosphorylated. It uses the same buffers as our Phospho-alpha Synuclein kit and enables the analysis of phosphorylated and total proteins from a single sample for a better understanding of the alpha Synuclein protein mediated pathogenesis.
The alpha Synuclein protein is involved in the developmnt of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson’s disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA)
Benefits
- SPECIFICITY
- PRECISION
- DATA NORMALIZATION
Total alpha Synuclein assay principle
The Total-alpha Synuclein assay quantifies the expression level of Synuclein in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer.
The Total-alpha Synuclein assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of Synuclein in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.
Total Synuclein 2-plate assay protocol
The 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before adding Total-Synuclein HTRF detection reagents.
This protocol enables the cells' viability and confluence to be monitored.
Total alpha Synuclein 1-plate assay protocol
Detection of total alpha Synuclein with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required.
This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.
Alpha synuclein level, quantified by HTRF, is more abundant in Hela cells compared to SH-SY5Y
Hela and SH-SY5Y cells were seeded and incubated until confluence at 37°C, 5% CO2.
After cell lysis, the lysates were serially diluted. The total protein content was then quantified using BCA reagent, while alpha Synuclein was quantified with the HTRF Total alpha Synuclein cellular kit.
Specifically quantification of the alpha Synuclein, not the beta Synuclein
HTRF total alpha Synuclein does not detect the beta-Synuclein protein.
Parkinson's disease pathway and a-synuclein role
Alpha-Synuclein is a prominent component of intracellular fibrillary aggregates in the brains of patients suffering from synucleinopathies.
This protein represents a key molecular hallmark for Parkinson’s disease, dementia with Lewy bodies, or multiple system atrophy. The extensive phosphorylation of alpha synuclein on Ser129 is also associated with a pathological event.
Changes in CREB and ERK phosphorylation levels after beta-amyloid treatment - Application Notes
Detection of human tau protein aggregation
For researcher working on PD or AD - Application Notes
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Exploring LRRK2 phosphorylation with HTRF® assays
LRRK2 kit, a game changer in neuroscience - Posters
Exploring Synuclein phosphorylation and aggegation with HTRF assays
Alpha Synuclein kits, perfect tools to study synucleinopathy - Posters
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
Product Insert a-Syn total Kit / 6FNSYPEG-6FNSYPEH
6FNSYPEG-6FNSYPEH - Product Insert
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Assays for neurosciences research
Advance your research on neurodegenerative diseases - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn total Kit / 6FNSYPEG
6FNSYPEG - Safety Data Sheet
Safety Data Sheet (DEU) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn total Kit / 6FNSYPEH
6FNSYPEH - Safety Data Sheet
Safety Data Sheet (DEU) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn total Kit / 6FNSYPEY
6FNSYPEY - Safety Data Sheet
Plate Reader Requirement
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