Phospho-BTK (Tyr223) cellular kit
Simple and robust detection kit for Phospho BTK & Total BTK
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This HTRF kit monitors cellular BTK expression levels and can be used as a normalization assay with the phospho-BTK (Tyr223) kit.
The Total BTK cellular assay monitors total BTK and is used as a normalization assay with our phospho-BTK kits with respect to steady state levels. BTK is involved in the B cell receptor and the NF kappa B signaling pathways, and has an important role in primary immunodeficiency, in particular for X-linked agammaglobulinemia (XLA) disease.
Using the two-plate assay protocol, human B-cell lymphoma line Raji cells were plated at 200,000 cells/ well in a half 96 well plate in 20 µl of FBS-free culture medium. After treatment for 20 minutes at 37°C (in triplicate) with 10µL of pervanadate at different concentrations , cells were lysed with 10 µL of supplemented lysis buffer #1 (4X) for 30min at RT under gentle shaking. 16 µL of lysate were transferred into a 384-well low volume white microplate with 4 µL of the pre-mix detection antibodies. The HTRF signal was recorded after an overnight incubation.
Human Erythroleukemic K562 cells and Human lymphoma B-cell Raji cells were plated respectively at 100 kcells/well & 200 kcells/well under 20µl of FBS-free culture medium in a half-96 well plate. After treatment for 2 hours at 37°C with 5 µL of increasing concentrations of Ibrutinib, 5 µL of 50µM pervanadate were added for 20 min at 37°C. Cells were then lysed with 10 µL of supplemented lysis buffer #1 (4X) for 30min at RT under gentle shaking. 16 µL of lysate were transferred into a 384-well low volume white microplate with 4 µL of the pre-mix detection antibodies. The HTRF signal was recorded after an overnight incubation.
Human Erythroleukemic K562 cells and Human lymphoma B-cell Raji cells were plated respectively at 100 kcells/well & 200 kcells/well under 20µl of FBS-free culture medium in a half-96 well plate. After treatment for 2 hours at 37°C with 5 µL of increasing concentrations of P505-15, 5 µL of 50µM pervanadate were added for 20 min at 37°C. Cells were then lysed with 10 µL of supplemented lysis buffer #1 (4X) for 30min at RT under gentle shaking. 16 µL of lysate were transferred into a 384-well low volume white microplate with 4 µL of the pre-mix detection antibodies. The HTRF signal was recorded after an overnight incubation.
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
PROTAC assays design at-a-glance
Streamlined set-up and access to multiple read-out options for research in oncology - Application Notes
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Product Insert BTK total Kit / 63ADK064PEG-63ADK064PEH
63ADK064PEG-63ADK064PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
An innate and adaptive immunity recap
Insight into the diversity of cells & signaling pathways - Guides
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Improve PROTAC Targeting Kinase Discovery
Application note describing how HTRF binding kits can help assessing PROTAC targeting kinase - Application Notes
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (ELL) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (ITA) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (SPA) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) BTK total Kit / 63ADK064PEG
63ADK064PEG - Safety Data Sheet
Safety Data Sheet (DEU) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (ELL) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (ITA) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (SPA) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) BTK total Kit / 63ADK064PEH
63ADK064PEH - Safety Data Sheet
Safety Data Sheet (DEU) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (ELL) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (ITA) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (SPA) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
Safety Data Sheet (ENG-US) BTK total Kit / 63ADK064PEY
63ADK064PEY - Safety Data Sheet
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