Advanced phospho-ERK (Thr202/Tyr204) cellular kit
Simple, all-in-one kit for robust detection of Phospho-ERK.
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This HTRF kit monitors the cellular ERK expression level and can be used as a normalization assay for the phospho-ERK kit.
The Total-ERK1/2 assay is ideal for ERK normalization with the phospho-ERK kits. It is compatible with the buffers from the phospho or Advanced phospho-ERK kits, so the same lysate can be used for fast and easy analysis of the total and the phosphorylated protein populations.
This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Investigating kinase activity in a cellular context
HTRF cellular assays - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations
HTRF: One technology, many uses in a pre-clinical laboratory setting
Benefits and considerations of HTRF - Scientific Presentations
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
HTRF solutions for each cell type - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
In collaboration with Molecular Devices - Posters
PI3K/AKT/mTor translational control pathway - Posters
mechanism of action of EGFR inhibitors assessed in the pancreatic BXPC3 cell line - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
TCR signaling investigation with HTRF phospho assays
Study a pathway of interest in PBMC and T cells - Application Notes
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Product Insert ERK total Kit / 64NRKPET
64NRKPET - Product Insert
STING HTRF offer to bridge innate and adaptive immunity
cGAS-STING signaling pathway from A to Z - Brochures
Product Insert ERK total Kit / 64NRKPEG-64NRKPEH
64NRKPEG-64NRKPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
An innate and adaptive immunity recap
Insight into the diversity of cells & signaling pathways - Guides
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Neuroinflammation study combining hiPSC-derived astrocytes and HTRF
See published experiments and data demonstrating how immunoassays rise to the challenge of astrocyte studies in neuroinflammation research - Application Notes
On-demand webinar: Linking Neuroinflammation and Neurodegeneration
New insight into neuroinflammation research - Videos
Assays for autoimmunity research
Advance your research on autoimmune diseases - Flyers
Inhibition of FGFR Signaling Pathways Studied in Cancer Cell Lines Using HTRF
The knowledge and data necessary to understand the inhibition value of FGFR receptors involved in human cancers. HTRF technology enables the monitoring of the activity of the AZD4547 inhibitor on cell signal transduction in 3 cancer cell lines. - Application Notes
a convincing demonstration of the reliability of the AlphaLISA and HTRF KRAS portfolios to evaluate compound in vitro therapeutic profiles in a cellular context - Application Notes
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (ELL) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (ITA) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (SPA) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) ERK total Kit / 64NRKPEG
64NRKPEG - Safety Data Sheet
Safety Data Sheet (DEU) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (ELL) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (ITA) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (SPA) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) ERK total Kit / 64NRKPEH
64NRKPEH - Safety Data Sheet
Safety Data Sheet (DEU) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (FRA-FR) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (ITA) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (SPA) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (ENG-GB) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (ENG-US) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (ELL) ERK total Kit / 64NRKPET
64NRKPET - Safety Data Sheet
Safety Data Sheet (DEU) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (ELL) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (ITA) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (SPA) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
Safety Data Sheet (ENG-US) ERK total Kit / 64NRKPEY
64NRKPEY - Safety Data Sheet
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