Total GSK3 alpha cellular kit HTRF®
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Ease-of-use
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Highly specific
Overview
The Total GSK3a cellular assay is used as a normalization assay with the phospho-GSK3a kit. It is optimal for metabolic research. GSK3 is involved in PI3K/AKT signaling, Wnt/ß-Catenin, Hedgehog, Notch, glycogen synthesis, and cytoskeleton polymerization, which control protein synthesis, cell proliferation, migration, inflammation, immune response, glucose regulation, and apoptosis.
Benefits
- SPECIFICITY
- PRECISION
- DATA NORMALIZATION
Total-GSK3a assay principle
Total-GSK3a 2-plate assay protocol
Total-GSK3a 1-plate assay protocol
HTRF assay compared to Western Blot using phospho-GSK3a, total-GSK3a

GSK3 Simplified Pathway
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Product Insert GSK3 a total Kit / 64GTAPEG-64GTAPEH
64GTAPEG-64GTAPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (DEU) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (ELL) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (ITA) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (SPA) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) GSK3 a total Kit / 64GTAPEG
64GTAPEG - Safety Data Sheet
Safety Data Sheet GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (DEU) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (ELL) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (ITA) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (SPA) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) GSK3 a total Kit / 64GTAPEH
64GTAPEH - Safety Data Sheet
Safety Data Sheet (DEU) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (ELL) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (ITA) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (SPA) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Safety Data Sheet (ENG-US) GSK3 a total Kit / 64GTAPEY
64GTAPEY - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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