cAMP - Gs assay kits

Discover the benefits of a solution tailored for Gs coupled GPCR research

Discover 2 Cisbio kits that enable you to quantify cAMP (cyclic-AMP) accumulation in response to Gs-coupled GPCR activation: cAMP - Gs Dynamic and cAMP - Gs HiRange.

Based on HTRF technology, these kits contain all the reagents you need to quantify cAMP, ensuring that you benefit from a complete tool for your needs.

Cisbio’s cAMP kits are best suited for measuring cAMP produced by Gs-coupled GPCR, leveraging an optimized combination of:

  • Sensitivity
  • Robustness
  • Precision
  • Ease-of-use

New to GPCR research? Cisbio has compiled a document section with all the resources you will need to get your project started, including reference documents, expert webinars, and insider tips.

Download cAMP guide - agonists of Gi/o
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Assays principle

Cisbio cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.

Native cAMP produced by cells competes with d2-labeled cAMP for binding to monoclonal anti-cAMP Eu3+ cryptate.

The protocol is simple and straightforward, with just two incubation steps:

  • Cell stimulation by the target compounds
  • cAMP detection using HTRF reagents

What to expect at the bench

Product Specifications

cAMP-Gs dynamic

Use this optimized kit to study your most challenging and precious cellular models.

Designed to produce results with minimal cell consumption, this kit features the lowest nanomolar IC50. Left-shifted IC50 enables you to quantify lower cAMP concentrations, significantly reducing the number of cells required.

cAMP-Gs HiRange

If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs. The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.

Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.

  S/B IC20 nM IC50 nM IC80 nM
cAMP - Gs dynamic >14 0.91 4.07 18.1
cAMP - Gs HiRange >28 4.27 22.8 121

 

ATP Cross-reactivity

No ATP cross-reactivity up to 10 mM

No ATP cross-reactivity up to 1 mM

No ATP cross-reactivity up to 1 mM

Product validation

HEK293 cells expressing the endogenous β2 adrenergic receptor were treated with the isoproterenol agonist for 45min. Isoproterenol was well characterized, displaying the right potency in good correlation with the literature.

HEK293 cells at 7,500 cells/well (optimal cellular concentration situated in the dynamic range of the kit) were treated with a serial concentration of isoproterenol, a beta 2 adrenergic receptor full-agonist. The EC80-EC90 was determined to choose the optimal agonist concentration to use for the antagonist mode experiment. Fitting the serial dilution of the antagonist allowed IC50 calculation for the compounds (potency) which was in perfect correlation with the literature.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
cAMP - Gs Dynamic kit - 1,000 tests62AM4PEB
cAMP - Gs Dynamic kit- 20,000 tests62AM4PEC
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cAMP - Gs Dynamic kit - 100,000 tests62AM4PEJ
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cAMP - Gs HiRange kit - 1,000 tests62AM6PEB
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cAMP - Gs HiRange kit - 20,000 tests62AM6PEC
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cAMP - Gs HiRange kit - 100,000 tests62AM6PEJ
-

Companion products

DescriptionCat. noProduct insertMSDS
cAMP - Gs Dynamic & Gi standard62AM4CDA
-
cAMP - Gs Dynamic & Gi 10 standards62AM4CDX
-
cAMP - Gs HiRange standard62AM6CDA
-
cAMP - Gs HiRange 10 standards62AM6CDX
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IBMX - Phosphodiesterase inhibitor - 40 µl62AMXADA
-
IBMX - Phosphodiesterase inhibitor - 500 µl62AMXADC
-
Forskolin - Adenylyl cyclase activator - 40 µl62AMYADA
Forskolin - Adenylyl cyclase activator - 800 µl62AMYADC
-
Lysis & Detection Buffer 1 - 13 ml62CL1FDD
Lysis & Detection Buffer 2 - 200 ml62CL2FDF
Stimulation Buffer 1 (5X) - 8 ml64SB1FDC
Stimulation Buffer 1 (5X) - 100 ml64SB1FDD

Literature