HTRF® profiling and screening

HTRF assays run by HTRF experts

The first step in finding a drug candidate is usually to find a potential hit within a library.
Cisbio completes your work effectively by helping you follow-up, validate, and screen your hits.

Compound testing

Validating hits: We perform dose-response experiments to determine IC50/EC50 of a small compound collection with biochemical or cell-based assays, typically in a 384-well format.Assay examples include:

  • GPCR: cAMP / IP-One, receptor-ligand binding
  • Cell signaling assays
  • cGMP for Phosphodiesterase (PDE) readout

Screening: Cisbio can screen small compound collections with any of our patented HTRF platforms, like IP-One for GPCR compound validation after Calcium flux in primary screening.

Biological testing

Comparability, stability, and lot release testing are important for developing biosimilars. Cisbio bioanalytical services for comparability studies include Fc binding on FcγR cell-based assays, and an FcRn assay for testing biosimilars. Take advantage of our extensive experience with biosimilar bioanalysis, including Bevacizumab, Trastuzumab, Rituximab, Etanercept, Adalimumab, Cetuximab, and Pertuzumab.

 

Multiparametric cytokine profiling

How it works

Select your panel of interest from among our cytokines.
We provide you with a sample preparation protocol and associated material (buffers).
We perform all cytokine dosage upon sample reception.
Results are presented and discussed, along with a complete report.

Multiparametric detection of IL10, IL6 and TNF in THP1 cell supernatant

Cell culture: 100 000 cells per well were seeded in a 384-well format.

After 6h of treatment with LPS (0.5 µg/ml), cell supernatant were taken and IL10, IL6 and TNF were detected according to Cisbio protocol.

Right:
Concentration (pg/ml) of IL10, IL6, and TNF from the same THP1 cell supernatant.

Biosimilar testing services

How it works

You choose the variants to be tested and provide your samples.
Cisbio performs the binding assays and provides you with scientific data and a complete analysis.

Fc binding assay on CD16A

In this example biotherapeutic antibodies (and controls) were run at different concentrations to determine their affinity for CD16 (V158) receptor. After incubating the antibodies with cells expressing CD16A (V158) for 2h, IC50's were calculated and plotted (right).

Samples tested with CD16AV1

Binding assays

Assay in action

Our binding service is based on the Cisbio Tag-lite product portfolio.
Competition assays are run with any unlabeled ligands to determine binding efficiency and constants.

How it works

You choose your receptor of interest from our validated list and provide a panel of unlabeled ligands to be tested.
We provide the cells expressing the labeled receptor and the matching labeled ligand to perform the binding assay.
Once completed, you receive the scientific data, along with a complete analysis.

M4 receptor ligand binding assay

In this example a competition assay was run with acetylcholine and the effect of a positive allosteric modulator could be evaluated at 2 concentrations (0.4 and 0.04µM).

Comparison can be done with a reference ligand to study potential allosteric modulators