Phospho- and total SLP-76 assays for monitoring T lymphocyte activation
SLP-76 is a cytoplasmic adapter protein, expressed in multiple hematopoietic lineages. Phospho-SLP-76 creates a scaffold on which key signaling complexes are built, and plays a critical role in T-cell development and activation. Following TCR engagement, SLP-76 is phosphorylated by Zap-70 and translocates to the plasma membrane where it promotes the assembly of a multi protein complex. This complex participates in the activation, survival, and proliferation of T-Lymphocytes.
Cisbio's cell-based homogeneous HTRF® phospho-SLP-76 (Ser376) immunoassay enables the quantitative detection of SLP-76 phosphorylated on Serine 376. The HTRF® total SLP-76 assay kit is designed for the quantitative detection of total SLP-76, phosphorylated and unphosphorylated, to normalize the phosphorylation status of SLP-76 with respect to its steady-state level in cells.
The buffers of both HTRF® phospho- and total SLP-76 assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample.