Sensitive detection of GPCR-mediated Erk1/2 phosphorylations in various cellular models with the HTRF Advanced Phospho-Erk1/2 kit


Cisbio, Codolet, France

Cisbio offers a comprehensive line of HTRF® assays to study GPCR signaling pathways. These include GPCR second messenger assays for cAMP and IP1 investigations as well as kits for phosphoprotein assays for downstream readouts. This application note shows the ability of the Advanced Phospho-ERK1/2 (Thr202/Tyr204) kit to detect ERK1/2 phosphorylation in different cellular models. The data demonstrate that the Advanced Phospho-ERK1/2 assay provides very sensitive detection of ERK1/2 phosphorylation mediated by G-protein coupled receptor (GPCR) activation in both generic and physiological cellular models with overexpressed and endogenous GPCRs.

Main readouts for GPCR signaling pathway investigations including P-ERK.

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