Start from pre-cloned plasmids

The largest selection of tagged cell surface receptor constructions

Cisbio offers a collection of full-length human GPCR SNAP-Tag plasmids. All GPCR genes are cloned in expression plasmids directly downstream of a CMV promoter and are ready for protein expression in your preferred mammalian cells.

STEP 1 Plasmid selection

Select your full-length human GPCR and TRK plasmids

Family Receptor Tag Plasmid - Part# Sequence
Adenosine
A2A Halo Tag PHTA2A TXT
Adrenoceptor beta1 SNAP PSNAPBETA1 TXT
beta2 SNAP PSNAPBD2 TXT
Anaphylatoxin C5L2 SNAP PSNAPC5L2 TXT
Apelin AGTRL1 SNAP PSNAPAGTRL1 TXT
Bombesin BB1 SNAP PSNAPBB1 TXT
BB2 SNAP PSNAPBB2 TXT
Chemokine CXCR4 SNAP PSNAPCXCR4 TXT
CCR5 SNAP PSNAPCCR5 TXT
Cholecystokinin CCK1 SNAP PSNAPCCK1 TXT
CCK2 SNAP PSNAPCCK2 TXT
Class A Orphans GPR1 SNAP PSNAPGPR1 TXT
Dopamine D1 SNAP PSNAPD1 TXT
D2 SNAP PSNAPD2 TXT
D3A SNAP PSNAPD3A TXT
Endothelin ETA SNAP PSNAPETA TXT
ETB SNAP PSNAPETB TXT
Ghrelin GHSR1 A SNAP PSNAPGHSR1A TXT
Glucagon GIPR SNAP PSNAPGIPR TXT
GLP1 SNAP PSNAPGLP1 TXT
Histamine H1 SNAP PSNAPH1 TXT
H3 SNAP PSNAPH3 TXT
Muscarinic M1 SNAP PSNAPM1 TXT
M2 SNAP PSNAPM2 TXT
M4 SNAP PSNAPM4 TXT
M5 SNAP PSNAPM5 TXT
Opioid Delta opioid SNAP PSNAPDOP TXT
Kappa opioid SNAP PSNAPOPRK1 TXT
Orexin OX1 SNAP PSNAPOX1 TXT
OX2 SNAP PSNAPOX2 TXT
Parathyroid PTH1 SNAP PSNAPPTH1 TXT
Serotonin 5HT1A SNAP PSNAP5HT1A TXT
5HT1B SNAP PSNAP5HT1B TXT
5HT2C SNAP PSNAP5HT2C TXT
Somatostatin SSTR4 SNAP PSNAPSSTR4 TXT
Tachykinin NK1 SNAP PSNAPNK1 TXT
Vasopressin/Oxytocin V2 SNAP PSNAPV2 TXT
Receptor Tag Plasmid - Part# Sequence
A2A Halo Tag PHTA2A TXT
beta1 SNAP PSNAPBETA1 TXT
beta2 SNAP PSNAPBD2 TXT
C5L2 SNAP PSNAPC5L2 TXT
AGTRL1 SNAP PSNAPAGTRL1 TXT
BB1 SNAP PSNAPBB1 TXT
BB2 SNAP PSNAPBB2 TXT
CXCR4 SNAP PSNAPCXCR4 TXT
CCK1 SNAP PSNAPCCK1 TXT
CCK2 SNAP PSNAPCCK2 TXT
GPR1 SNAP PSNAPGPR1 TXT
D1 SNAP PSNAPD1 TXT
D2 SNAP PSNAPD2 TXT
D3A SNAP PSNAPD3A TXT
ETA SNAP PSNAPETA TXT
ETB SNAP PSNAPETB TXT
GHSR1 A SNAP PSNAPGHSR1A TXT
GIPR SNAP PSNAPGIPR TXT
GLP1 SNAP PSNAPGLP1 TXT
H1 SNAP PSNAPH1 TXT
H3 SNAP PSNAPH3 TXT
MC4 SNAP PSNAPMC4 TXT
MC5 SNAP PSNAPMC5 TXT
M1 SNAP PSNAPM1 TXT
M2 SNAP PSNAPM2 TXT
M4 SNAP PSNAPM4 TXT
M5 SNAP PSNAPM5 TXT
Delta opioid SNAP PSNAPDOP TXT
Kappa opioid SNAP PSNAPOPRK1 TXT
OX1 SNAP PSNAPOX1 TXT
OX2 SNAP PSNAPOX2 TXT
PTH1 SNAP PSNAPPTH1 TXT
5HT1A SNAP PSNAP5HT1A TXT
5HT1B SNAP PSNAP5HT1B TXT
5HT2C SNAP PSNAP5HT2C TXT
5HT4 SNAP PSNAPHTR4 TXT
Somatostatin SNAP PSNAPSSTR4 TXT
NK1 SNAP PSNAPNK1 TXT
V2 SNAP PSNAPV2 TXT

 

STEP 2 Cell transfection

Use standard transfection techniques, to transiently express the SNAP-GPCR of interest in your cell line

download

STEP 3 Cell labeling

SNAP-tag®, is a small fusion tag that covalently interacts with specific substrates. SNAP-tag allows specific and covalent labeling of any protein of interest. Cells are provided unlabeled and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available from the Cisbio catalog in 4 different sizes.


How to label cell surface receptors
using Tag-lite® technology

STEP 4 Binding assay

The Tag-lite® receptor ligand binding assay is a homogeneous alternative to radio ligand binding assays for HTS and compound profiling.

Saturation binding assay (Kd determination)

Mesurement of total and non-specific binding of increasing ligand concentrations under equilibrium conditions.


How to implement a saturation binding
assay using Tag-lite® technology

Competitive binding assay (Ki determination)

Measurement of a compound's dissociation constant.


How to implement a competitive binding
assay using Tag-lite® technology

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
SNAP/CLIP lab medium 5X - 100 mlLABMED
pHaloTag-A2A Adenosine receptorPHTA2A
pSNAP-5HT1A 5-Hydroxytryptamine 1APSNAP5HT1A
pSNAP-5HT1B 5-Hydroxytryptamine 1BPSNAP5HT1B
pSNAP-5HT2C 5-Hydroxytryptamine 2CPSNAP5HT2C
pSNAP-Apelin receptor (AGTRL1)PSNAPAGTRL1
pSNAP-BB1 Bombesin receptorPSNAPBB1
pSNAP-BB2 Bombesin (GRP) receptorPSNAPBB2
pSNAP-Beta2 adrenoreceptorPSNAPBD2
pSNAP-Beta1 adrenoreceptorPSNAPBETA1
pSNAP-C5L2 Anaphylatoxin receptorPSNAPC5L2
pSNAP-CCK1 Cholecystokine type APSNAPCCK1
pSNAP-CCK2 Cholecystokine type BPSNAPCCK2
pSNAP-CCR5 Chemokine receptorPSNAPCCR5
pSNAP-CXCR4 Chemokine receptorPSNAPCXCR4
pSNAP-D1 Dopamine receptor type 1PSNAPD1
pSNAP-D2 Dopamine receptor type 2PSNAPD2
pSNAP-D3A Dopamine receptorPSNAPD3A
pSNAP-Delta opioid receptorPSNAPDOP
pSNAP-ETA Endothelin receptor type APSNAPETA
pSNAP-ETB Endothelin receptor type BPSNAPETB
pSNAP-Ghrelin receptor type APSNAPGHSR1A
pSNAP-GIPR Glucagon receptorPSNAPGIPR
pSNAP-GLP1 Glucagon receptorPSNAPGLP1
pSNAP-GPR1 receptorPSNAPGPR1
pSNAP-H1 Histamine receptorPSNAPH1
pSNAP-H3 Histamine receptorPSNAPH3
pSNAP-Acetylcholine muscarinic receptor M1PSNAPM1
pSNAP-Acetylcholine muscarinic receptor M2PSNAPM2
pSNAP-Acetylcholine muscarinic receptor M4PSNAPM4
pSNAP-Acetylcholine muscarinic receptor M5PSNAPM5
pSNAP-NK1 Tachykinin substance P receptorPSNAPNK1
pSNAP-OPRK1 Opioid receptorPSNAPOPRK1
pSNAP-OX1 Orexin receptorPSNAPOX1
pSNAP-OX2 Orexin receptorPSNAPOX2
pSNAP-PTH1 Parathyroid receptor type 1PSNAPPTH1
pSNAP-SSTR4 Somatostatin receptorPSNAPSSTR4
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pSNAP-V2 Arginine vasopressin receptor 2PSNAPV2
SNAP-Lumi4-Tb - 2 nmolesSSNPTBC
SNAP-Lumi4-Tb - 5 nmolesSSNPTBD
SNAP-Lumi4-Tb - 5x5 nmolesSSNPTBG
SNAP-Lumi4-Tb - 100 nmolesSSNPTBX

Literature