Run your ligand binding assay directly with Cisbio cells and their matching fluorescent ligands for non-radioactive, fluorescent saturation, and competitive binding assays. For optimal efficiency, our cells are ready-to-use, pre-transfected, and pre-labeled.
Start from ready to use, pre-transfected, pre-labeled cells
Saturation binding assay (Kd determination)
Competitive binding assay (Ki determination)
Find the best ligand/labeled cell combination for your assay
Literature
-
Flyers
Cell-based assays for neurosciences find_in_page
Cisbio Bioassays, Codolet, France
pdf GPCR research from A to Z find_in_page
Cisbio Bioassays, Codolet, France
pdf pdf Tag-lite® binding assays find_in_page
pdf -
Guides
Understanding GPCRs is the key to improved drug discovery find_in_page
Cisbio, Codolet, France
Download -
Application notes
HTRF Ligand Binding Assay for the Chemokine Receptor CXCR4 find_in_page
Cisbio, BioTek Instruments
pdf
Our Technology HTRF

HTRF® technology principle:
Truly homogeneous functional assays
FRET (Fluorescence Resonance Energy Transfer) is based on the transfer of energy between two fluorophores – a donor and an acceptor – when in close proximity. Molecular interactions between biomolecules can be assessed by coupling each partner with a fluorescent label and by detecting the level of energy transfer.