Human CCL2 (MCP1) kit
No-wash kit to quantify released Human CCL2 (MCP1)
Viral double-stranded RNA detection kit HTRF®
The dsRNA kit isdesigned to detect double-stranded RNA, a trait shared by all viruses and a sign of host-cell infection, in cell lysates.
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Ready-to-use
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Ease-of-use
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Cell-based
Overview
Double-stranded RNA (dsRNA) generated during genome replication is a commonality to many viruses. Traditional methods for detecting virus infection have relied on titering virus particles (plaque assay). These methods have proven lengthy, often taking place over several days. This assay detects the presence of dsRNA in cell lysates, a byproduct of viral replication activity, making it possible to obtain same-day results.
Benefits
- ALTERNATIVE TO THE PLAQUE ASSAY
- SAME-DAY RESULTS
- COMPATIBLE WITH MANY STRAINS
Assay principle
dsRNA is detected in a sandwich assay format using two antibodies, one labelled with Europium Cryptate (donor) and the second with d2 (acceptor). The detection principle is based on HTRF technology. When the dyes are in close proximity, the excitation of the donor with a light source (laser or flash lamp) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn fluoresces at a specific wavelength (665 nm). The two antibodies bind to the dsRNA present in the sample, thus generating FRET. Signal intensity is proportional to dsRNA concentration.
Assay Protocol
The assay protocol, using a 384-well small volume white plate or a Cisbio low volume 96 well plate (20 µL final), is described on the right. 10 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Detection of synthetic dsRNA
The signal was obtained on a range of concentrations of a synthetic dsRNA template diluted in the kit's lysis buffer. The synthetic dsRNA is used to mimic the dsRNA produced upon viral infection and subsequent genome replication.
Product Insert Viral dsRNA Kit / 64RNAPEG-64RNAPEH
64RNAPEG-64RNAPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Safety Data Sheet (DEU) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (ELL) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (ITA) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (SPA) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Viral dsRNA Kit / 64RNAPEG
64RNAPEG - Safety Data Sheet
Safety Data Sheet (DEU) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (ELL) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (ITA) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (SPA) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Viral dsRNA Kit / 64RNAPEH
64RNAPEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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